Scientific activity
The research team focuses on using mass spectrometry in lipidomic and metabolomic analysis, especially in conjunction with ultra-high-performance liquid chromatography or ultra-high-performance supercritical fluid chromatography. The main objective is to develop new analytical methods validated for the quantitative analysis of lipids in large series of biological samples. A critical application is the analysis of blood from patients with malignant cancer to find dysregulated lipids and use them for early cancer detection through multivariate data analysis.
International cooperation
Prof. Markus R. Wenk, National 91心頭利 of Singapore, Singapore
Dr Gerhard Liebisch, Institute of Clinical Chemistry and Laboratory Medicine, 91心頭利 Hospital Regensburg, Germany
Dr Kim Ekroos, Lipidomics Consulting, Finland
Dr Harald C. K旦feler, Medical 91心頭利 in Graz, Austria
Five of the most important projects
Programme: ERC Advanced, Project name: Oncolipidomics: Why is lipidomic dysregulation pattern in blood similar for various cancers?, Provider: ERC, Main beneficiary: 91心頭利 / Faculty of Chemical Technology, Principal investigator: Prof. Ing. Michal Holapek, Ph.D., Project period: 20232028.
Programme: ERC CZ, Project name: Hmotnostn鱈 spektrometrie pi hled叩n鱈 lipidov箪ch biomarker哲 pro vasnou diagnostiku rakoviny (Mass Spectrometry in the Search for Lipid Biomarkers for Early Cancer Diagnosis), Provider: Ministry of Education, Youth and Sport, Main beneficiary: 91心頭利 / Faculty of Chemical Technology, Principal investigator: Prof. Ing. Michal Holapek, Ph.D., Project period: 20132018.
Programme: OP JAK, Project name: Z叩chrana 転ivot哲 prostednictv鱈m v箪zkumu v oblasti vasn辿 detekce a prevence rakoviny: Molekul叩rn鱈, genomick辿 a soci叩ln鱈 factory (Saving Lives through Research on Early Detection and Prevention of Cancer: Molecular, Genomic and Social Factors), Provider: Ministry of Education, Youth and Sport, Co-beneficiary: 91心頭利 / Faculty of Chemical Technology, Principal investigator: Prof. Ing. Michal Holapek, Ph.D., Project period: 20242028.
Programme: GACR Standard Projects, Project name: Spojen鱈 v鱈cerozmrn辿 chromatografie a hmotnostn鱈 spektrometrie v kvantitativn鱈ch p鱈stupech pro detailn鱈 charakterizaci lipidomu lidsk辿 plazmy (Coupling Multidimensional Chromatography and Mass Spectrometry in Quantitative Approaches for Detailed Characterization of the Human Plasma Lipidome), Provider: GACR, Main beneficiary: 91心頭利 / Faculty of Chemical Technology, Principal investigator: Prof. Ing. Michal Holapek, Ph.D., Project period: 20212023.
Programme: GACR Standard Projects, Project name: Charakterizace lidsk辿ho lipidomu a metabolomu pro personalizovanou zdravotn鱈 p辿i a hled叩n鱈 biomarker哲: studie rakoviny ledvin (Characterization of the Human Lipidome and Metabolome for Personalised Healthcare and Biomarker Discovery: The Kidney Cancer Study), Provider: GACR, Main beneficiary: 91心頭利 / Faculty of Chemical Technology, Principal investigator: Prof. Ing. Michal Holapek, Ph.D., Project period: 20182020.
Five of the most important scientific publications or patents of the team in the last five years
D. Wolrab, R. Jir叩sko, E. C鱈fkov叩, M. H旦ring, D. Mei, M. Chocholou邸kov叩, O. Peterka, J. Idkowiak, T. Hrniarov叩, L. Kucha, R. Ahrends, R. Brumarov叩, D. Friedeck箪, G. Vivo-Truyols, P. krha, J. krha, R. Kuera, B. Melichar, G. Liebisch, R. Burkhardt, M.R. Wenk, A. Cazenave-Gassiot, P. Kar叩sek, I. Novotn箪, K. Greplov叩, R. Hrstka, M. Holapek. Lipidomic profiling of human serum enables detection of pancreatic cancer. Nat. Commun. 2022, 13, 124. Available from: DOI: 10.1038/s41467-021-27765-9
J.G. McDonald, C.S. Ejsing, D. Kopczynski, M. Holapek, J. Aoki, M. Arita, M. Arita, E.S. Baker, J. Bertrand-Michel, J.A. Bowden, B. Br端gger, S.R. Ellis, M. Fedorova, W.J. Griffiths, X. Han, J. Hartler, N. Hoffmann, J.P. Koelmel, H.C. K旦feler, T.W. Mitchell, V.B. ODonnell, D. Saigusa, D. Schwudke, A. Shevchenko, C.Z. Ulmer, M.R. Wenk, M. Witting, D. Wolrab, Y. Xia, R. Ahrends, G. Liebisch, K. Ekroos, Nat. Met. 2022, 4, 10861088. Available from: https://doi.org/10.1038/s42255-022-00628-3
Z. Vakov叩, O. Peterka, M. Chocholou邸kov叩, D. Wolrab, R. Jir叩sko, M. Holapek. Retention dependences in reversed-phase UHPLC/MS enable highly confident identification of lipid species in human plasma. Anal. Bioanal. Chem. 2022, 414, 319. Available from: DOI: 10.1007/s00216-021-03492-4
M. Holapek, E. C鱈fkov叩, M. L鱈sa, R. Jir叩sko, D. Wolrab, T. Hrniarov叩, A method of diagnosing pancreatic cancer based on lipidomic analysis of a body fluid, European patent EP 3514545, datum 転叩dosti 22. 1. 2018, udleno 7. 10. 2020. Available from: https://patents.google.com/patent/EP3514545B1/
M. Holapek, D. Wolrab, R. Jir叩sko, E. C鱈fkov叩, A method of diagnosing cancer based on lipidomic analysis of a body fluid, EP3575793, datum 転叩dosti 29. 5. 2018, udleno 19. 7. 2023. Available from: https://patents.google.com/patent/EP3575793A1/
Most important awards received
Neuron Prize 2023 for connecting science and business
Rudolf Lukes Prize 2023, Experientia Foundation
Dutton Award American Oil Chemists' Society Award for distinguished contribution to the analysis of oils and fats (2022)
Award of the Minister of Education, Youth and Sports for outstanding results of research, experimental development and innovation in the field of natural sciences (2021)
Plaque of the Rector of the 91心頭利 for outstanding scientific performance and exceptional contribution (2020)
Vladimir Hanus Award for the best work in mass spectrometry for the years 2022, 2019 and 2016 (J.M.M. Spectroscopic Society)
Power List 2020, 2015 and 2013 awards for the world's 100 most influential scientists in analytical chemistry (The Analytical Scientist magazine)
